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KMID : 0670820090140030133
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Korean Journal of Medical Mycology
2009 Volume.14 No. 3 p.133 ~ p.144
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Reidentification of Aspergillus spp. Isolated from Clinical Specimens of Patients Suspected as Pulmonary Aspergillosis in Korea
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Kim Dae-Ho
Kim Shin-Ok
Kim Sang-Jae
Kim Young-Kwon
Kim Sung-Hee
Hong Seung-Beom
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Abstract
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Korean Institute of Tuberculosis (KIT) collected 129 strains of Aspergillus spp. from clinical specimens of patient suspected as pulmonary Aspergillosis. On the basis of morphological characteristics, these strains were identified as A. fumigatus (62 strains), A. niger (37), A. flavus (26), A. versicolor (1), A. nidulans (1), A. clavatus (1) and Neosartorya fennelliae (1). These strains were re-identified according to recent Aspergillus classification system which is mainly based on molecular characters. The strains were grouped by Randomly Amplified Polymorphic DNA (RAPD). The representative strains from each group were sequenced with partial β-tubulin gene and compared with those of reference strains in the Aspergillus and were identified by the sequence. The identification was confirmed by morphological
examination. As the results, they are reidentified as A. fumigatus (58), A. niger (11), A. tubingensis (26), A. flavus (27), A. sydowii (3), A. nidulans (1), A. clavatus (1), Neosartorya fennelliae (1), N. fischeri (1). This is also the first report of A. tubuingensis in clinical field in Korea.
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KEYWORD
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Plumonary Aspergillosis, Aspergillus, Identification, β-tubulin
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